The act reduces by $264,268 an appropriation for the 2025-26 state fiscal year from the general fund to the department of natural resources division of parks and wildlife (division) for the reintroduction of gray wolves. The act requires the state treasurer to transfer a corresponding amount from the general fund to the Colorado health insurance affordability enterprise cash fund on September 1, 2025. The act also prohibits the division from using the money appropriated from the general fund in state fiscal year 2025-26 (appropriated money) for the acquisition and reintroduction of gray wolves. The division may use the appropriated money to assist owners of livestock in preventing and resolving conflicts between gray wolves and livestock and to pay fair compensation to owners of livestock for any losses of livestock caused by gray wolves. APPROVED by Governor August 28, 2025 EFFECTIVE August 28, 2025(Note: This summary applies to this bill as enacted.)