The bill aims to restrict submitting data derived from animal testing for product or substance applications, except when no non-animal test method is available or a waiver is granted. Limited exemptions apply for existing data, foreign regulatory requirements, and insufficiency of existing data. Entities must minimize animal harm when no non-animal method is available. Civil penalties of up to $10,000 may apply for violations. Agencies are to issue guidance on non-animal test method use, publish annual progress reports, and make related information publicly available. The bill defines key terms and covers multiple agencies, seeking to promote alternative test methods and reduce animal testing.